What is ONT-based plasmid sequencing?
▼
Plasmid sequencing using Oxford Nanopore Technology (ONT) is a long-read sequencing approach that enables accurate characterization of entire plasmids in a single run.
What plasmid sizes can be sequenced?
▼
ONT sequencing supports plasmids from small (<15 kb) to very large constructs (>300 kb).
What are the advantages of ONT?
▼
Full-length sequencing, no primer walking, faster turnaround, and better resolution of complex regions.
What type of samples can I submit?
▼
Extracted plasmid DNA or bacterial cultures (liquid or colonies).
What DNA quality is required?
▼
A260/A280 ~1.7–1.8, A260/A230 2.0–2.2, and contaminant-free DNA.
How much DNA is required?
▼
Typically 20–50 µl with concentration 20–300 ng/µl.
Can ONT detect mutations?
▼
Yes, including point mutations, insertions, deletions, and structural variations.
What is the turnaround time?
▼
Typically 2–7 working days depending on sample complexity.
What deliverables will I receive?
▼
FASTQ data, assembled sequences, QC reports, and optional analysis.
Can you sequence mixed samples?
▼
Not recommended. Submit single clonal plasmids for best results.
Do I need sequencing primers?
▼
No. ONT does not require primers.
Is ONT suitable for large plasmids?
▼
Yes, especially for large, complex, and repetitive plasmids.
How should I ship samples?
▼
Use sealed tubes, leak-proof packaging, ice packs, and proper labeling.
Do you provide data analysis support?
▼
Yes, including assembly, annotation, and variant detection.