Prepare Your Samples

We accept samples as resuspended colonies or liquid cultures.

🧬 Resuspended Colonies

• Pick one large, well-isolated colony (>1 mm diameter)
• Resuspend in 10 µl nuclease-free water
• Use a 1.5 ml sterile microcentrifuge (Eppendorf) tube

🧪 Liquid Culture

• Grow bacterial culture to high density (saturated culture)
• Send 30 ml culture in a 50 ml conical tube
• Avoid buffered media such as Terrific Broth (TB)

Tips for Low Copy Plasmids

• Use larger colonies (>1 mm) for resuspension samples
• Ensure visibly saturated cultures for liquid samples

Packaging Instructions

• Seal tubes securely (parafilm recommended)
• Place samples in a leak-proof container
• Ship with an ice pack
• Clearly label samples and include correct shipping address